Opportunities at Bio@SNS

1) Three year post-doc position to work on pain signal transduction at Scuola Normale (Pisa, Italy)

We are seeking a highly motivated and enthusiastic Post Doc to perform signal transduction studies  related to molecules of the Nerve Growth Factor family in the field of pain. The position will be in the group of Prof. Antonino Cattaneo at the Bio@SNS Laboratory of Scuola Normale Superiore in Pisa. The group has pioneered technologies in the field of recombinant antibodies and  has developed a number of  therapeutic candidates for chronic pain. The group has a considerable experience in developing innovative strategies to address experimental questions in the  field of neurodegeneration.

In this EU funded three-years project (PAINCAGE),, the SNS team will investigate the signaling properties of mutant forms of NGF and of TrkA receptors related to the HSAN IV and HSAN V Human genetic desease. Two Knock-in mouse models for HSAN IV and HSAN V are being developed and characterized. the differences in activation of NGF receptor by mutant form of NGF and TrkA receptors in different cellular models related to two human genetic diseases, the Hereditary Sensory and Autonomic Neuropathies type IV and V.  .

The candidate must have significant experience in state of the art molecular and cell biology techniques and an excellent practice in  performing signal transduction studies. The candidate should be able to work independently and to interact with other members of the group and of the other teams in the Project.

Interested candidates should directly email their curriculum vitae, a brief statement of research experience and career goals, and names of two referees to  antonino.cattaneo@sns.it

2) Three year post-doc position to work on animal models of neuropathic and osteoarthritic pain at Scuola Normale (Pisa, Italy)

We are seeking a highly motivated and enthusiastic Post Doc to perform signal transduction studies  related to molecules of the Nerve Growth Factor family in the field of pain. The position will be in the group of Prof. Antonino Cattaneo at the Bio@SNS Laboratory of Scuola Normale Superiore in Pisa. The group has pioneered technologies in the field of recombinant antibodies and  has developed a number of  therapeutic candidates for chronic pain. The group has a considerable experience in developing innovative strategies to address experimental questions in the  field of neurodegeneration.

In this EU funded three-years project, (PAINCAGE), the SNS team will derive mouse models of osteoarthritic pain, neuropathic pain in diabetic mice and mouse models related to two human genetic diseases, the Hereditary Sensory and Autonomic Neuropathies type IV and V in which pain perception is lost. The candidate will perform behavioral studies to assess pain, cognition and attentional deficits and test the efficacy of potential therapeutic agents of the NGF family.

The candidate must have significant experience in manipulating and managing laboratory animals, excellent practice in behavioral studies and in histological and biochemical techniques. The candidate should be able to work independently and to interact with other members of the group and of the other teams in the Project.

Interested candidates should directly email their curriculum vitae, a brief statement of research experience and career goals, and names of two referees to  antonino.cattaneo@sns.it

3) Two years post-doc position to work on the structural characterization of protein involved in chronic pain (Scuola Normale Superiore  – Pisa, Italy and Institute of Christallography at CNR, Trieste, Italy)

We are seeking a highly motivated and enthusiastic post-doc to perform structural studies on molecules of the Nerve Growth Factor (NGF) receptor family and on their antibodies. The position will be in the group of Antonino Cattaneo at Scuola Normale Superiore (Pisa) and of Doriano Lamba at the Institute of Christallography in Trieste. The Joint groups have a considerable experience in the structural characterization of proteins relevant for neurodegeneration and chronic pain. The experimental work will be performed mostly in Trieste.

In this EU funded three-years project (PAINCAGE) the SNS/CNR team will perform the biophysical and structural characterization of complexes between the antiTrkA MNAC13 antibodies in Fab format and the two immunoglobulin-like domains d4-d5 of TrkA and between the antiNGF αD11 antibodies in Fab Format and NGF. Moreover the team will perform the structural characterization of the NGFR100 HSAN V mutant.

The candidate must have significant experience in the biochemical purification of proteins in their biophysical characterization and in their christallographic structural characterization. The candidate should be able to work independently and to interact with other member of the team.

Interested candidates should directly email their curriculum vitae, a brief statement of research experience and career goals, and names of two referees to  antonino.cattaneo@sns.it

4) Two year post-doc position to work on synaptic intrabodies at Scuola Normale (Pisa, Italy)

We are seeking a highly motivated and enthusiastic Post Doc to perform molecular biology work with recombinant antibodies derived from libraries in yeast cells in the group of Prof. Antonino Cattaneo at the Bio@SNS Laboratory of Scuola Normale Superiore in Pisa. The group has pioneered the technology of intracellular antibodies (intrabodies) and its use for subcellularly precise protein silencing and has considerable experience in developing innovative strategies to address experimental questions in the cell biology of neurotrophin actions and neurodegeneration.

 

In this two-years project (NEUROANTIBODIES), funded by the EU Human Brain Project, the team  will derive recombinant antibodies against transynaptic proteins of the neuroligin-neurexin families, and express them as intrabodies targeted to synapses, to investigate their functional role in the bidirectional control of synaptic transmission. Collected data will be used to implement computational models of trans-synaptic signaling and receptor kinetics.

 

The candidate must have significant experience in state of the art molecular and cell biology techniques and an excellent understanding of neuronal and synaptic cell biology, in order to design targeting strategies for synaptic intrabodies and validate the constructs experimentally. The candidate should be able to work independently and to interact with other members of the group and of the other teams in the Project.

 

Interested candidates should directly email, before May 5th 2014, their curriculum vitae, a brief statement of research experience and career goals, and names of two referees to  antonino.cattaneo@sns.it

 

5) Two year post-doc position to work on intracellular antibody libraries in yeast at Scuola Normale (Pisa, Italy)

We are seeking a highly motivated and enthusiastic Post Doc to perform molecular biology work with recombinant antibodies derived from libraries in yeast cells in the group of Prof. Antonino Cattaneo at the Bio@SNS Laboratory of Scuola Normale Superiore in Pisa. The group of Antonino Cattaneo has pioneered the Intellectual Antibody Capture Technology (IACT) that allows the effective isolation of antibodies directly from gene sequences with no manipulation of the corresponding proteins and has a considerable experience in developing innovative strategies to address experimental questions in the cell biology of neurotrophin actions and neurodegeneration.

In this two-years project (NEUROANTIBODIES), funded by the EU Human Brain Project, the team will derive recombinant antibodies against transynaptic proteins of the neuroligin-neurexin families, and express them as intrabodies targeted to synapses  to investigate their functional role in the bidirectional control of synaptic transmission. Collected data will be used to implement computational models of trans-synaptic signaling and receptor kinetics. The candidate must have significant experience in state of the art molecular biology techniques, with particular regard to (either or both) i) the construction and handling of large molecular repertoires of antibodies and ii) genetic and two-hybrid screens in yeast cells. The candidate should be able to work independently and to interact with other members of the group and of the other teams in the Project. Interested candidates should directly email their curriculum vitae, a brief statement of research experience and career goals, and names of two referees to  antonino.cattaneo@sns.it